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immunofluorescence staining for p21  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc immunofluorescence staining for p21
    Immunofluorescence Staining For P21, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 3052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+if+staining/p21+Waf1%2FCip1+Rabbit+mAb/10__1007_slash_s12668___026___02800___5-59-0-4
    Average 97 stars, based on 3052 article reviews
    immunofluorescence staining for p21 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Lymphotropic Virotherapy Engages DC and High Endothelial Venule Inflammation to Mediate Cancer In Situ Vaccination
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585), MECA-79 (Biolegend #120802), and ISG15 (Invitrogen #703132) sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI). .. Where indicated, HCR-FISH was performed on the stained slides for imaging of PVSRIPO (-)strand vRNA with specific probes (Molecular Instruments Reagents) (Supplementary Table 1).

    Article Title: Injectable immunoregulatory hydrogels sequentially drive phenotypic polarization of macrophages for infected wound healing.
    Article Snippet: The concentration of cytokines in supernatants were detected using Elisa assay kit (Dakewe, China), including IL-1β, IL-10, TNF-α, and TGF-β. .. Immunofluorescence (IF) staining for CD86/CD206 (19589/24595, Cell signaling technology, USA) was implemented. ..

    Article Title: LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1.
    Article Snippet: In vivo apoptosis was evaluated using the TUNEL assay with the In Situ Cell Death Detection Kit TMR red (Cat. 12156792910; Roche) following the manufacturer’s manual. .. Macrophage infiltration was evaluated using immunofluorescence (IF) staining with the primary antibody against the F4/80 antigen (#30325, 1:400; Cell Signaling Technology, Boston, MA, USA). ..

    Article Title: Gap junctions are essential for murine primordial follicle assembly immediately before birth.
    Article Snippet: .. Antibodies were prepared as follows: for IHC staining, DDX4 (mouse, ab4300, 1:400; Abcam) primary antibody or BrdU (mouse, NBP2-29414, 1:400; Imgenex, CO, USA) primary antibody was diluted in PBS; for immunofluorescence (IF) staining, DDX4 (1:400) and MKI67 (rabbit, 9129S, 1:400; CST, MA, USA) primary antibodies were diluted in antibody dilution buffer (ADB) (3% BSA, 1% normal donkey serum in www.reproduction-online.org Tris-buffered saline) as the primary antibody cocktail. ..

    Article Title: Lymphotropic Virotherapy Induces DC and High Endothelial Venule Inflammation, Promoting the Antitumor Efficacy of Intratumor Virus Administration
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571). .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571).

    Staining:

    Article Title: Lymphotropic Virotherapy Engages DC and High Endothelial Venule Inflammation to Mediate Cancer In Situ Vaccination
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585), MECA-79 (Biolegend #120802), and ISG15 (Invitrogen #703132) sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI). .. Where indicated, HCR-FISH was performed on the stained slides for imaging of PVSRIPO (-)strand vRNA with specific probes (Molecular Instruments Reagents) (Supplementary Table 1).

    Article Title: Injectable immunoregulatory hydrogels sequentially drive phenotypic polarization of macrophages for infected wound healing.
    Article Snippet: The concentration of cytokines in supernatants were detected using Elisa assay kit (Dakewe, China), including IL-1β, IL-10, TNF-α, and TGF-β. .. Immunofluorescence (IF) staining for CD86/CD206 (19589/24595, Cell signaling technology, USA) was implemented. ..

    Article Title: LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1.
    Article Snippet: In vivo apoptosis was evaluated using the TUNEL assay with the In Situ Cell Death Detection Kit TMR red (Cat. 12156792910; Roche) following the manufacturer’s manual. .. Macrophage infiltration was evaluated using immunofluorescence (IF) staining with the primary antibody against the F4/80 antigen (#30325, 1:400; Cell Signaling Technology, Boston, MA, USA). ..

    Article Title: Gap junctions are essential for murine primordial follicle assembly immediately before birth.
    Article Snippet: .. Antibodies were prepared as follows: for IHC staining, DDX4 (mouse, ab4300, 1:400; Abcam) primary antibody or BrdU (mouse, NBP2-29414, 1:400; Imgenex, CO, USA) primary antibody was diluted in PBS; for immunofluorescence (IF) staining, DDX4 (1:400) and MKI67 (rabbit, 9129S, 1:400; CST, MA, USA) primary antibodies were diluted in antibody dilution buffer (ADB) (3% BSA, 1% normal donkey serum in www.reproduction-online.org Tris-buffered saline) as the primary antibody cocktail. ..

    Article Title: Lymphotropic Virotherapy Induces DC and High Endothelial Venule Inflammation, Promoting the Antitumor Efficacy of Intratumor Virus Administration
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571). .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571).

    Incubation:

    Article Title: Lymphotropic Virotherapy Engages DC and High Endothelial Venule Inflammation to Mediate Cancer In Situ Vaccination
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585), MECA-79 (Biolegend #120802), and ISG15 (Invitrogen #703132) sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI). .. Where indicated, HCR-FISH was performed on the stained slides for imaging of PVSRIPO (-)strand vRNA with specific probes (Molecular Instruments Reagents) (Supplementary Table 1).

    Article Title: Lymphotropic Virotherapy Induces DC and High Endothelial Venule Inflammation, Promoting the Antitumor Efficacy of Intratumor Virus Administration
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571). .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571).

    Blocking Assay:

    Article Title: Lymphotropic Virotherapy Engages DC and High Endothelial Venule Inflammation to Mediate Cancer In Situ Vaccination
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585), MECA-79 (Biolegend #120802), and ISG15 (Invitrogen #703132) sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI). .. Where indicated, HCR-FISH was performed on the stained slides for imaging of PVSRIPO (-)strand vRNA with specific probes (Molecular Instruments Reagents) (Supplementary Table 1).

    Article Title: Lymphotropic Virotherapy Induces DC and High Endothelial Venule Inflammation, Promoting the Antitumor Efficacy of Intratumor Virus Administration
    Article Snippet: .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571). .. For immunofluorescence (IF) staining of CD11c (Cell Signaling #97585, RRID:AB_2800282), MECA-79 (Biolegend #120802, RRID:AB_493555), and ISG15 (Thermo Fisher #703132, RRID: AB_3666159), sections were fixed in 4% paraformaldehyde (20min), permeabilized (Triton X-100, 0.5%), incubated in blocking buffer (donkey serum, 5%), incubated in primary antibody (1:200 dilution; 24h, 4°C), rinsed (TBS, 3x), incubated with secondary anti-species antibody (donkey anti-rat; Invitrogen #A48272, RRID:AB_2893138; 2h, 20°C), rinsed (TBS, 3x), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D3571).

    Immunohistochemistry:

    Article Title: Gap junctions are essential for murine primordial follicle assembly immediately before birth.
    Article Snippet: .. Antibodies were prepared as follows: for IHC staining, DDX4 (mouse, ab4300, 1:400; Abcam) primary antibody or BrdU (mouse, NBP2-29414, 1:400; Imgenex, CO, USA) primary antibody was diluted in PBS; for immunofluorescence (IF) staining, DDX4 (1:400) and MKI67 (rabbit, 9129S, 1:400; CST, MA, USA) primary antibodies were diluted in antibody dilution buffer (ADB) (3% BSA, 1% normal donkey serum in www.reproduction-online.org Tris-buffered saline) as the primary antibody cocktail. ..

    Saline:

    Article Title: Gap junctions are essential for murine primordial follicle assembly immediately before birth.
    Article Snippet: .. Antibodies were prepared as follows: for IHC staining, DDX4 (mouse, ab4300, 1:400; Abcam) primary antibody or BrdU (mouse, NBP2-29414, 1:400; Imgenex, CO, USA) primary antibody was diluted in PBS; for immunofluorescence (IF) staining, DDX4 (1:400) and MKI67 (rabbit, 9129S, 1:400; CST, MA, USA) primary antibodies were diluted in antibody dilution buffer (ADB) (3% BSA, 1% normal donkey serum in www.reproduction-online.org Tris-buffered saline) as the primary antibody cocktail. ..



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    Image Search Results


    Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) Immunofluorescence staining of OCN and OPN in BO after the different treatments. H) Western blot of RUNX2 and OCN expression. I,J) Quantification of RUNX2 and OCN. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Hydrogel-integrated osteogenic microtissues promote repair of infected intervertebral defects through sequential immunomodulation

    doi: 10.1016/j.mtbio.2026.103657

    Figure Lengend Snippet: Osteogenic response supported by hydrogel under conditional inflammatory environment of RAW264.7 macrophages. A, B) Schematic illustrations of the inflammatory culture systems established using conditioned medium from LPS-stimulated RAW264.7 macrophages to evaluate osteogenic differentiation of rBMSCs (A) and osteogenic bone microtissues (BO) (B) . C, E) Representative ALP)staining and quantitative analysis of ALP activity on day 7. D, F) Representative ARS staining and quantitative analysis of mineral deposition on day 14. G) Immunofluorescence staining of OCN and OPN in BO after the different treatments. H) Western blot of RUNX2 and OCN expression. I,J) Quantification of RUNX2 and OCN. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Article Snippet: Osteogenic protein expression in bone microtissues was assessed by immunofluorescence staining for OCN (ABclonal, A6205, 1:200) and OPN (ABclonal, A23658, 1:200) and by western blotting for RUNX2(Abcam, ab192256,1:1000), OCN (ABclonal, A6205, 1:1000), and GAPDH (ABclonal, AC001, 1:10,000).

    Techniques: Staining, Analysis, Activity Assay, Immunofluorescence, Western Blot, Expressing

    Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunohistochemical staining, Staining, Saline, Expressing, Immunofluorescence, Generated, Standard Deviation

    Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunofluorescence, Staining, Expressing, Generated, Standard Deviation